Fig. 5

Map-based cloning of ES2 and identification of mutated site in es2. a ES2 was preliminarily mapped between markers B2–10 and B2–11 on chromosome 2. b Fine mapping of ES2. The ES2 locus was fine mapped to a 116.7 kb region between markers ID2–3 and ID2–4. c Eighteen putative ORFs are located in the 116.7 kb region. d Gene structure of the candidate gene LOC_Os02g32370. Scale bar =200 bp. The black rectangle represents the exon. The point mutation of G to A on the exon led to amino acid replacement of Gly with Glu. e Comparison of sequence chromatograms around the mutated site between the wild-type WYG7 and es2