Fig. 4

PCR-RE assay and Sanger sequencing of single clones after transferring plasmid DNA into banana protoplasts. The PCR-amplified fragment of edited target 3 (t3ko) or its wild type (t3wt) and edited target 4 (t4ko) or its wild type (t4wt) were digested by Eco47I (dig) or were not digested (undig). Gene edited fragments were amplified with the DNA from transformed protoplasts, while wild-type target fragments were amplified with the DNA from untransformed protoplasts. M indicates DNA marker (a). Sanger sequencing of single clones of undigested fragments from Fig. 4a (b). The blue letters indicate the PAM sequence; red letters signify mutations with base insertions; a red ‘-‘means a mutation with a base deletion