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Fig. 3 | BMC Plant Biology

Fig. 3

From: Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

Fig. 3

Schematic representation of the vectors and targets implemented in this study. Diagrams of pUbi-Cas9, sgRNA plasmid and Cas12a vector. Ubip, ubiquitin promoter from Zea mays. Pvubip, ubiquitin promoter from Phaseolus vulgaris. CaMVter, termination sequence of cauliflower mosaic virus (a). Details of two targets on the PDS gene in banana. The positions of target 3 and target 4 on the gene and the endonuclease sites are illustrated (b). Red letters indicate PAM sequence

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