Full-length transcriptome analysis of Phytolacca americana and its congener P. icosandra and gene expression normalization in three Phytolaccaceae species

Table 4 Primers for RT-qPCR analysis

Gene name	Gene description	Primer sequence (5′-3′)	Length (bp)	PCR Efficiency (%)	R²
Tubulin	Tubulin 8	F: GTAAGGAAGCCGAGAATTG	181	103	0.984
Tubulin	Tubulin 8	R: TCAACAACAGTGTCAGAGA	181	103	0.984
EF1-α	Elongation factor 1-alpha	F: TGAAGAAGGTCGGATACAAT	194	95	0.993
EF1-α	Elongation factor 1-alpha	R: GTAGACATCCTGGAGTGG	194	95	0.993
GAPHD	Glyceraldehyde-3-phosphate dehydrogenase	F: TGGTGCTAAGAAGGTTATTATC	198	104	0.986
GAPHD	Glyceraldehyde-3-phosphate dehydrogenase	R: GAGTGAACGGTGGTCATA	198	104	0.986
EF2	Elongation factor 2	F: GTATCACCATCAAGTCAACTG	194	99	0.999
EF2	Elongation factor 2	R: ACAATCAACCACAACAAGG	194	99	0.999
18S rRNA	18S rRNA	F: ACTTCCTCTTCTCGTATCATT	193	99	0.993
18S rRNA	18S rRNA	R: TGTTCAGCATAGACTGTGA	193	99	0.993
β-Actin	Actin-7	F: ATGCTATCCTTCGTCTGG	180	105	0.996
β-Actin	Actin-7	R: TACTCTTGGCTGTCTCTG	180	105	0.996
28S rRNA	28S rRNA	F: TACGATTGGTTACGGACAT	191	102	0.983
28S rRNA	28S rRNA	R: TTCTCATCAACAACAGCATAT	191	102	0.983

ISSN: 1471-2229