Fig. 6From: Full-length transcriptome analysis of Phytolacca americana and its congener P. icosandra and gene expression normalization in three Phytolaccaceae speciesPairwise variation analyzed by geNorm to determine the optimal number of reference genes for accurate normalization. A threshold value of 0.15 was suggested for valid normalization. If the value of Vn/n + 1 (pairwise variation) is less than 0.15, then n reference genes in combination are recommended for gene normalization. If the value of Vn/n + 1 is more than 0.15, then Vn + 1/n + 2 should be taken into account. Pam: P. americana; Pic: P. icosandra; Pac: P. acinosa; LSRF: different tissues of leaves, stems, roots and flowers; GS: germinating seeds of these three plant species; F: flowers of these three plant species; LSR: different tissues of leaves, stems and roots; I: infested by S. litura of third instarBack to article page