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Fig. 6 | BMC Plant Biology

Fig. 6

From: Full-length transcriptome analysis of Phytolacca americana and its congener P. icosandra and gene expression normalization in three Phytolaccaceae species

Fig. 6

Pairwise variation analyzed by geNorm to determine the optimal number of reference genes for accurate normalization. A threshold value of 0.15 was suggested for valid normalization. If the value of Vn/n + 1 (pairwise variation) is less than 0.15, then n reference genes in combination are recommended for gene normalization. If the value of Vn/n + 1 is more than 0.15, then Vn + 1/n + 2 should be taken into account. Pam: P. americana; Pic: P. icosandra; Pac: P. acinosa; LSRF: different tissues of leaves, stems, roots and flowers; GS: germinating seeds of these three plant species; F: flowers of these three plant species; LSR: different tissues of leaves, stems and roots; I: infested by S. litura of third instar

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