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Table 2 Effect of NaHS on endogenous hydrogen sulfide (H2S), non-protein thiols (NPTs), and glutathione (GSH) content in leaves and roots of iron-deficient Glycine max plants. The 3-w-old Glycine max seedlings were treated with the nutrition solution containing 1 μM Fe(III)-EDTA or 50 μM Fe(III)-EDTA with or without 100 μM NaHS for 15 days. Error bars represents the mean ± SE. Different letters indicate significant differences with P < 0.05. –Fe, 1 μM Fe; −Fe + NaHS, seedlings treated with 100 μM NaHS and 1 μM Fe; +Fe, 50 μM Fe; +Fe + NaHS, seedlings treated with 100 μM NaHS and 50 μM Fe

From: Hydrogen sulphide alleviates iron deficiency by promoting iron availability and plant hormone levels in Glycine max seedlings

Treatment Endogenous H2S content (μmol g− 1 FW) NPTs (μmol g− 1 FW) GSH (nmol g− 1 FW)
Leaf Root Leaf Root Leaf Root
-Fe 0.046 ± 0.013 b 0.013 ± 0.004 C 7.56 ± 0.20 b 3.74 ± 0.10 B 28.56 ± 5.71 c 164.4 ± 9.71 B
-Fe + NaHS 0.123 ± 0.014 a 0.075 ± 0.010 A 8.95 ± 0.29 a 4.88 ± 0.44 A 149.5 ± 15.6 b 187.1 ± 6.62 A
+Fe 0.056 ± 0.007 b 0.029 ± 0.002 B 7.96 ± 0.64 ab 4.05 ± 0.27 B 162.3 ± 10.7 b 166.2 ± 10.8 B
+Fe + NaHS 0.119 ± 0.010 a 0.098 ± 0.017 A 8.37 ± 0.25 a 5.28 ± 0.26 A 240.2 ± 15.7 a 182.4 ± 5.34 A