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Table 4 Transmission of pigs-1 mutation in self-pollinated pistils

From: AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis

Selfed parent

Observed No. of progeny

 

BastaR

BastaS

Lethal

Χ2

P-value

+/+; BastaR/+

130

43

0

0.001

0.97

pigs-1/+

151

153

0

52.01

< 0.001

pigs-1/+; GFP-PIGS #15–3

512

256

166

101.4

< 0.001

pigs-1/+; GFP-PIGS #7–25

152

74

65

40.22

< 0.001

pigs-1/+; GFP-PIGS #25–6

402

154

112

44.61

< 0.001

  1. Basta herbicide-resistant (BastaR) or susceptible (BastaS) progeny. Basta resistance is linked with the T-DNA that is inserted into either the AtPIGS gene (pigs-1 mutant) (Fig. 2a) or in an unknown location in the genome of the +/+; BastaR/+ (positive control line) [21]
  2. χ2 was calculated based on the expectation of a 3:1 segregation of basta resistance to susceptibility in the progeny
  3. Seedling phenotypes scored according to images and descriptions in Fig. 3
  4. GFP-PIGS refers to three independent transformant T2 lines of pPIGS:GFP-PIGS