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Table 4 Transmission of pigs-1 mutation in self-pollinated pistils

From: AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis

Selfed parent Observed No. of progeny  
BastaR BastaS Lethal Χ2 P-value
+/+; BastaR/+ 130 43 0 0.001 0.97
pigs-1/+ 151 153 0 52.01 < 0.001
pigs-1/+; GFP-PIGS #15–3 512 256 166 101.4 < 0.001
pigs-1/+; GFP-PIGS #7–25 152 74 65 40.22 < 0.001
pigs-1/+; GFP-PIGS #25–6 402 154 112 44.61 < 0.001
  1. Basta herbicide-resistant (BastaR) or susceptible (BastaS) progeny. Basta resistance is linked with the T-DNA that is inserted into either the AtPIGS gene (pigs-1 mutant) (Fig. 2a) or in an unknown location in the genome of the +/+; BastaR/+ (positive control line) [21]
  2. χ2 was calculated based on the expectation of a 3:1 segregation of basta resistance to susceptibility in the progeny
  3. Seedling phenotypes scored according to images and descriptions in Fig. 3
  4. GFP-PIGS refers to three independent transformant T2 lines of pPIGS:GFP-PIGS