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Table 3 Seedling lethality of pigs-1/pigs-1; pPIGS:GFP-PIGS on MS plates

From: AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis

Selfed parent

Observed No. of progeny

% Lethal

Normal

Lethal

pigs-1/+

346

0

0

pigs-1/+; GFP-PIGS #15–3

315

78

19.85

pigs-1/+; GFP-PIGS #7–25

140

32

18.60

pigs-1/+; GFP-PIGS #25–6

324

127

28.16

  1. Selfed seeds were placed onto MS plates containing no antibiotic or basta
  2. Seedling phenotypes were scored according to images and descriptions in Fig. 3
  3. GFP-PIGS refers to three independent transformant T2 lines of pPIGS:GFP-PIGS