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Fig. 3 | BMC Plant Biology

Fig. 3

From: AtPIG-S, a predicted Glycosylphosphatidylinositol Transamidase subunit, is critical for pollen tube growth in Arabidopsis

Fig. 3

Lethal seedlings segregated in the progeny of selfed pigs-1/+, pPIGS:GFP-PIGS. a-c Light micrographs of seedlings from selfed seeds of pigs-1/+, pPIGS:GFP-PIGS line #25–6 grown on MS plates supplemented with basta for 14 days in constant light. a Basta-resistant pigs-1/+, pPIGS:GFP-PIGS seedlings had well developed true green leaves and prolific root, root hairs, and lateral root growth. b Basta-susceptible +/+, pPIGS:GFP-PIGS seedlings had minimal to no true leaves or root hair and lateral root development. c Lethal pigs-1/pigs-1, pPIGS-PIGS-GFP seedlings were small, showed aberrant pale cotyledon growth, and lacked radicle or roots. *Images shown were taken using a Zeiss Axiovert 100 microscope with either an 8x objective, Bar = 5 mm (a) or 50x objective, Bar = 5 mm (b-c). d-f Gene model of the (d) pPIGS:GFP-PIGS construct, (e) endogenous AtPIG-S gene, and (f) pigs-1 T-DNA insertion. Primers used in the PCR-based genotyping of each of these three DNAs are indicated. g-h PCR-based genotyping using gDNA isolated from basta-resistant, basta-susceptible, and lethal seedling progeny of PIGS-GFP T2 lines #15–3 and #26–6 for either the presence of the pigs-1 T-DNA using primers 822 + 1886 (g) or for the presence of the endogenous AtPIG-S or GFP-PIGS gene using primers 2474 + 1885 (h). Expected size for endogenous AtPIG-S is 1896 bp and expected size for GFP-PIGS is 2586 bp. Original, un-cropped gels are provided in Additional file 6. N, water control; C, Columbia-0 gDNA; R, basta-resistant; S, basta susceptible; L, lethal seedlings. *All primer sequences listed in Additional Table 1

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