Fig. 1

Flow chart of the analysis procedure using the Ilastik-Fiji method. Eight rosettes for control or treated rosettes were scanned either on black or white background (a), rosettes are individualized, and the total area of each rosette is estimated using Photoshop (b). A selection of individual rosettes is imported to Ilastik (c) and used to train the program (d), to distinguish mite damage (red), from healthy rosette (green) and background (yellow). All the images are processed and exported as 8-bit (e). The previous images are imported into Fiji and the damaged areas extracted and exported as black and white images (f). The damaged area is calculated for treated and control rosettes (g). Control rosettes are then used to correct the damage area from mite-treated plants. Black and white scale bars indicate 1 cm