Fig. 5From: A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow guna. Isolation of IE of emmer wheat ‘Runo’ from immature caryopse. b. Isolated IEs placed scutellum-side up on the induction medium. c. Imbibed mature seeds with cutting on ME. d. Morphogenic callus from ME (21 days of culture) before the removal and the transfer onto the pre-culture mediumBack to article page