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Fig. 3 | BMC Plant Biology

Fig. 3

From: A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow gun

Fig. 3

Analysis for the inheritance of GFP fluorescence in T1 progeny. a. Four variants of GFP fluorescence in T1 seeds, left to right: the seed displaying the expression in embryo and endosperm, the seed without GFP expression in embryo showing endosperm fluorescence, the seed demonstrating fluorescence in T1 embryo, but lacking it in the endosperm, the seed that did not inherit the expression of GFP gene. b. Inheritance of GFP fluorescence in T1 seedlings of a primary transgenic plant, 3 days of in vitro culture of isolated embryos. Tissues were photographed under white light or UV light using the GFP filter set (EX BP 470/40, BS FT 495, EM LP 550)

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