Fig. 2From: A competence of embryo-derived tissues of tetraploid cultivated wheat species Triticum dicoccum and Triticum timopheevii for efficient and stable transgenesis mediated by particle inflow gunExample of Southern blot analyses of genomic DNAs from PCR- positive T0 transgenic plants of emmer wheat ‘Runo’ generated after biolistic-mediated transformation using IE-derived explants (labeled as PI) and ME-derived explants (labeled as PM); genomic as well as plasmid control DNA was digested with HindIII and the membrane was probed using the 510-bp Ubi1-BAR probe; lane P, psGFP-BAR (1.0 ng); lane WT, negative control representing DNA from untransformed ‘Runo’; under the gel, the inheritance of GFP expression in T1 seedlings of T0 primary plant is indicatedBack to article page