Fig. 9
Phenotypic and physiological analysis of transiently transgenic (Csa1G085390 and Csa7G071440) and negative control (pART27:GFP) plants under salt and drought stresses and normal condition (CK) after 4 h treatment. a Phenotypic comparison of transiently transgenic and negative control plants under salt and drought stresses. Corresponding water treatment was performed as negative control treatment (CK). Measurements of Water loss rate b, Hydrogen peroxide (H2O2) content f and malondialdehyde (MDA) g content in above treated leaves, respectively. Different letter indicates significant differences at the level of 0.05. c Stomata observation in transiently transgenic and negative control plants’ leaves. 30 leaves were observed for each plant and each treatment. d Quantification of stomatal apertures in the leaves of the transiently transgenic and control plants. Different letter indicates significant differences at the level of 0.05. e H2O2 detection by 3,3′-diaminobenzidine (DAB) staining in the leaves of the transiently transgenic and control plants . Scale bar: 5 cm. The photos were taken by Junliang Yin and the figure was drawn by Junliang Yin