Skip to main content
Fig. 9 | BMC Plant Biology

Fig. 9

From: SMRT and Illumina RNA sequencing reveal novel insights into the heat stress response and crosstalk with leaf senescence in tall fescue

Fig. 9

Quantitative real time PCR (qRT-PCR) validation and function analysis of differentially expressed transcription factors. a Relative expression of NAM-B2 (i1_LQ_TF3rd_c8869/f1p0/1379), NAC029 (i1_HQ_TF3rd_c26766/f2p2/1431), NAM-B1 (i1_HQ_TF3rd_c44692/f2p0/1636), HsfA2d-1 (i1_LQ_TF3rd_c31016/f1p0/1406), HsfA2d-2 (i1_LQ_TF3rd_c80591/f1p0/1429), and HsfA2a (i3_HQ_TF3rd_c12860/f2p0/3466). b Membrane damages in agro-infiltrated Nicotiana. benthamiana plants with NAC029 (i1_HQ_TF3rd_c26766/f2p2/1431), NAM-B1 (i1_HQ_TF3rd_c44692/f2p0/1636), and HsfA2a (i3_HQ_TF3rd_c12860/f2p0/3466) after 1 day at 45 °C by electrolyte leakage assay compared with agro-infiltrated plants with empty vector. Asterisks indicated significant difference with wild type at high temperature as estimated using Student’s t-test (* P < 0.05, ** P < 0.01). Error bars represent the standard error of the mean (n = 3)

Back to article page
\