Fig. 4
From: Stimulation of homologous recombination in plants expressing heterologous recombinases
Intrachromosomal recombination (ICR) frequency in homozygous lines. a Six-week-old seedlings of the control N1DC4 and homozygous lines expressing recombinases were stained for GUS activity and the number of blue spots per seedling was scored. b Pollen of the controls wild type (WT) and N1DC4 and two homozygous lines expressing DMC1 in N1DC4 background were stained for GUS activity to detect blue pollen (arrow, scale bar = 100 μm). c ICR events (blue pollen) were scored for the control N1DC4 and different homozygous lines expressing recombinases. The average value of ICR frequency in N1DC4 control was (0.134 ± 0.04)× 10− 4. A-C, RecA-2A-RuvC construct. Data correspond to an average of 3–12 plants (3 flowers/plant) and error bars indicate standard errors