Fig. 2
From: Stimulation of homologous recombination in plants expressing heterologous recombinases
Test of plasmid constructs using in vitro transcription and translation. a An example of a construct containing multiple coding sequences (RecA, RecG and RuvC) in a single open reading frame (arrow) cloned in pGEM®-T Easy vector (Promega) is shown. During translation, the 2A sequence of foot and mouth disease virus (black box) allows the production of the individual proteins including their nuclear localisation signal (hatched box). b TNT® wheat germ lysate was performed with plasmid DNA of pRecA-2A-RuvC (A-C), pRecA-2A-RecG-2A-RuvC (A-G-C), pRad52-2A-Rad51 (R52-R51) and pRad52-2A-DMC1-2A-Rad51 (R52-D-R51). Arrows indicate individual products