Fig. 6

qRT-PCR validation of the GWAS and RNA-seq results. Expression of six candidate genes in the low-temperature-resistant line Zao 8–3 (referred to as 55) and the low-temperature-sensitive line Ji 853 (referred to as 102). Expression analysis was conducted on embryos that were collected at 2 h and 4 h under low (10 °C) and normal (25 °C) temperature conditions, respectively. * and ** indicate significance at P < 0.05 and P < 0.01, respectively