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Fig. 6 | BMC Plant Biology

Fig. 6

From: Seed characterization and early nitrogen metabolism performance of seedlings from Altiplano and coastal ecotypes of Quinoa

Fig. 6

Molecular phylogenetic analyses of low and high affinity nitrate transporters in C. quinoa. Phylogenetic optimal trees of nucleotide coding sequences of nitrate transporter homologues of C. quinoa, other two species of the Chenopodiaceae family (S. oleracea and B. vulgaris), and A. thaliana. The evolutionary history was inferred using the Maximum-Likelihood method based on the Tamura-Nei model. In the phylogenetic analysis of Low Affinity Transporter (LAT) homologues (a), the tree with the highest log likelihood (− 21,210.48) is shown. In the phylogenetic analysis of high affinity transporters (HATs) (b), the tree with the highest log likelihood (− 29,578.42) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Joining and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. A discrete Gamma distribution was used to model evolutionary rate differences among sites. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 23 (a) and 40 (b) nucleotide sequences. All positions with less than 95% site coverage were eliminated. Accession number for each sequence is indicated. Gene homologues characterized by gene expression in this study are contained in boxes. Evolutionary analyses were conducted in MEGA7

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