Fig. 5

Gene expression of candidate photoperiod and flowering time genes in the Mtphya-1 mutant and R108 in LD and SD. Relative transcript abundance was measured in the fully expanded trifoliate leaves of 14-d-old wild-type R108 and Mtphya-1 homozygous seedlings. Relative gene expression was measured by qRT-PCR with normalization to PP2A. Data are the mean ± se of three biological replicates and relative to the highest value. Tissues were harvested 2 h after dawn. Different letters (a,b,c) indicate significant differences, while the same letter(s) on a bar indicate no significant differences using the two-way ANOVA test [multiple pairwise comparisons adjusted for false discovery rate (FDR); α = 0.05]. Tissues were harvested 2 h after dawn