Diversity and evolution of cytochrome P450s of Jacobaea vulgaris and Jacobaea aquatica

BMC Plant Biology

Table 1 Summary of Illumina sequencing and assemblies for two J. vulgaris and two J. aquatica sets

Sets ^a	Total paired-end clean reads	Total assembled trinity transcripts	Transcript length range (nt ^b)	GC content (%)	Contig N50 ^c (nt)	Average contig length (nt)	Reads mapped ^d (%)
Jv1*	19,725,242	152,286	301–13,238	39.37	1253	936	84.69
Jv2	36,359,675	142,213	301–13,269	39.31	1530	1132	83.25
Ja1*	20,306,518	118,936	301–15,708	39.27	1461	1082	91.57
Ja2	27,505,944	130,365	301–13,309	41.23	1441	1062	87.41

^a Jv1 consisted of the pooled shoots and roots of 59 individuals of J. vulgaris. Jv2 was composed of shoots from five tissue culture derived plants of J. vulgaris treated with MeJA and five mock treated individuals. Ja1 and Ja2 were derived from the same seven individuals of J. aquatica, of which roots were included in Ja1 but not in Ja2
^b nt: nucleotide
^c Contig N50: length such that sequence contigs of this length or longer include half the bases of the Trinity assembly
^d Reads mapped: the percentage of properly paired reads mapped back to the Trinity transcriptome assembly by Bowtie2
* cDNA library was normalized before sequencing

ISSN: 1471-2229