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Table 1 Summary of PacBio transcripts and comparisons with genomes reported in 2017

From: Transcriptome analysis of heat stress and drought stress in pearl millet based on Pacbio full-length transcriptome sequencing

 

Pacbio

Varshney et al., 2017

 

Before correct

After correct

 

Subreads base(G)

17.89

  

Subreads number

6,842,837

  

Average subreads length

2615

  

N50(subreads)

2787

  

CCS

354,139

  

5′-primer

332,357

  

3′-primer

332,288

  

Poly-A

326,534

  

Full length

306,369

  

Flnc

303,627

  

Average flnc read length

2897

  

Consensus reads

132,488

  

Total_nucleotides

409,482,590

410,858,545

 

Total_number

132,488

132,488

 

Mean_length

3091

3102

1014.71

Min_length

200

201

 

Max_length

14,226

14,277

 

N50(consensus)

3288

3302

 

N90(consensus)

1999

2006

 

Number of transcripts

 

132,488

69,398

Number of Genes

 

64,878

38,579

Number of genes annotated

 

62,436(96.24%)

29,344 (76.06%)

Number of genes unannotated

 

2442(3.76%)

9235 (23.94%)