Table 1 Candidate genes (CGs) for Sclerotinia Head Rot resistance analysed in this study. CGs successfully amplified in the AMP are underlined. a Acronyms used throughout this article. Gene target, chromosome, Gene start and Gene end refers to the genes annotated in Badouin et al. (2017), available at heliagene.org
From: Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower
Source | Sunflower IDa | Reference | Fold change | p-value | Sequencing primers | Gene target | Gene start (bp) | Gene End (bp) | Chromosome |
|---|---|---|---|---|---|---|---|---|---|
Sclerotinia differential expression assay (Helianthus annuus) | HeAn_12562 | Ehrenbolger et al. (2013) [49] | 2.36 | 0.01 | F: 5’AGCCCAGTGTCAAATCAACC3’ | AtRLP48 | 85,297,951 | 85,299,659 | HanXRQChr10 |
R: 5′ TCCATACTTACGGTTCCACTCA 3′ | |||||||||
HeAn_12761 | Ehrenbolger et al. (2013) [49] | 0.31 | 0.001 | F: 5’ATGCAAGCATGTGAAGCAGT 3′ | PLY2 | 39,465,262 | 39,466,985 | HanXRQChr17 | |
R: 5’CCCCAAGCAACATGACTTTT 3′ | |||||||||
HeAn_28738 | Ehrenbolger et al. (2013) [49] | 2.95 | 0.001 | F: 5′ GGTGGACCATTTCATCAAGG 3′ | HanXRQChr14g0449241 | ######## | ######## | HanXRQChr14 | |
R: 5′ CGTGGGACAGCAACACTAAA 3′ | |||||||||
Hean_315 | Ehrenbolger et al. (2013) [49] | −2.5 | 0.052 | F: 5′ CCACTCAATCTCCCCAAAAA 3′ | HSP41 | 12,595,842 | 12,596,417 | HanXRQChr06 | |
R: 5’CACCTTCGCCACTTTAATCC 3′ | |||||||||
HeAn_35791 | Ehrenbolger et al. (2013) [49] | −2.78 | 0.001 | F: 5’CCGTCACCATTTTCACATTG3′ | HanXRQChr13g0415061 | ######## | ######## | HanXRQChr13 | |
R: 5’TAAATTTTGGGAACCGGACA3′ | |||||||||
HeAn_36589 | Ehrenbolger et al. (2013) [49] | −3.7 | 0.055 | F: 5’CTCCGCAACCTCCACTACAT 3’ | HSP21 | 84,168,138 | 84,168,620 | HanXRQChr03 | |
R: 5’GAGGCAGCTTCTCCACAGTC 3’ | |||||||||
HeAn_41021 | Ehrenbolger et al. (2013) [49] | 12.50 | 0 | F: 5′ CGTCTTCGTTGTTGCAATCT 3′ | HanXRQChr16g0506041 | 39,200,062 | 39,209,510 | HanXRQChr16 | |
R: 5’AGGAGGAGGATGAGGAGCAT 3′ | |||||||||
HeAn_41022 | Ehrenbolger et al. (2013) [49] | −4.55 | 0 | F: 5’CAGGCCAAAGTCGAGAATGT 3′ | ASF2 | 39,035,731 | 39,038,365 | HanXRQChr16 | |
R: 5′ CACAAGGGAAACACAAACACTG 3′ | |||||||||
Sclerotinia differential expression assay (Brassica napus) | G21 | Zhao et al. (2007) [34] AT3G17130 | 18.74 | 4.49E-5 | F: 5′ TGAAGGTCCATCTCCTCTTATTTC 3’ | HanXRQChr13g0395221 | 59,003,751 | 59,004,311 | HanXRQChr13 |
R: 5′ GTCGCTAAAGCTCCGTTCAC 3′ | |||||||||
G22 | Zhao et al. (2007) [34] | 18.03 | 4.82E-6 | F: 5′ GGAACCTTATCCACCCGAAT 3′ | HanXRQChr06g0170521 | 17,800,642 | 17,804,614 | HanXRQChr06 | |
AT4G03400 | R: 5′ GCAAATCTTTCTCGGTGTCC 3′ | ||||||||
G26 | Zhao et al. (2007) [34] | 14.92 | 9.72E-6 | F: 5′ CTGATGAGTGGTCGGAGGTT 3′ | HanXRQChr10g0292811 | 95,584,317 | 95,586,370 | HanXRQChr10 | |
AT3G10190 | R: 5′ CCATCATTGTCGGCGTCTA 3′ | ||||||||
G28 | Zhao et al. (2007) [34] AT1G19100 | 14.23 | 8.31E-7 | F: 5′ CAGGATAGTAGTCCCGATGGTG 3′ | HanXRQChr15g0463011 | 95,505 | 102,279 | HanXRQChr15 | |
R: 5′ TCAAAGGTTCCAGTCGCAAA 3′ | |||||||||
G30 | Zhao et al. (2007) [34] | 12.17 | 1.20E-7 | F: 5′ CCCGACACTTATTAAGACTCG 3′ | HanXRQChr13g0406751 | ######## | ######## | HanXRQChr13 | |
AT5G02140 | R: 5′ GCTGATCCTAGTCAACAACTGC 3′ | ||||||||
G33 | Zhao et al. (2007) [34] | 12.16 | 1.20E-5 | F: 5′ TTGCATTATCCTGATGTTATTATCTTG 3′ | HanXRQChr16g0509301 | 62,305,294 | 62,307,846 | HanXRQChr16 | |
AT3G19630 | R: 5′ TTGATGAATTATACCAACCTACCAAA 3′ | ||||||||
G34 | Zhao et al. (2007) [34] | 13.89 | 3.97E-7 | F: 5′ TAGGCGATCGATGCTCACTT 3′ | HanXRQChr14g0449241 | ######## | ######## | HanXRQChr14 | |
AT3G28390 | R: 5′ TCAAGGCGAGTAGGGTAAGC 3′ | ||||||||
Sclerotinia head and stem rot association mapping (Helianthus annuus) | HaGLP3 | Fusari et al. (2012) [17] | _ | _ | F: 5′ TCACATTCTCTTCATCCTATGCTC 3′ | HanXRQChr12g0360281 | 18,742,800 | 18,743,470 | HanXRQChr12 |
R: 5′ CCAGTTCCACCAAGAACAGC 3′ | |||||||||
HaGLP4 | Fusari et al. (2012) [17] | _ | _ | F: 5′ TGCTTGTAACCTCCTCCTCCT 3′ | AB19A | ######## | ######## | HanXRQChr10 | |
R: 5′ TGTGGGAAAAGCATGATGTC 3′ | |||||||||
HaGLP5 | Fusari et al. (2012) [17] | _ | _ | F: 5′ TCAAGTGCTATGGCTACTAATGATTT 3′ | HanXRQChr06g0181801 | 74,845,978 | 74,847,869 | HanXRQChr06 | |
R: 5’TCGATGTATAAATGTATGGTAAAAGAA 3′ | |||||||||
HaCOI_1 | Talukder et al. (2014) [18] | _ | _ | F: 5′ CCGATTTGCCACTGGATAAC 3’ | COI1 | ######## | ######## | HanXRQChr14 | |
R: 5′ ACACGCTGGATAGTCGTTCC 3’ | |||||||||
HaCP | Fusari et al. (2012) [17] | _ | _ | F: 5′ TGCTATTGATGCTGGCAGTT 3′ | CYSEP | ######## | ######## | HanXRQChr09 | |
R: 5′ AATCATGTTTCACATACCAAATCTT 3′ | |||||||||
HaDRP | Fusari et al. (2012) [17] | _ | _ | F: 5′ CAAAGGAAGCAACAACACCA 3′ | HanXRQChr01g0029501 | ######## | ######## | HanXRQChr01 | |
R: 5′ TCGCTGATTCGATACCCTTT 3′ | |||||||||
HaPAL | Fusari et al. (2012) [17] | _ | _ | F: 5′ TGTGGTCTTCAAATTCATTAATAACC 3′ | PAL1 | 62,564,693 | 62,568,081 | HanXRQChr07 | |
R: 5′ GGCCATTCCTAACAGGATCA 3′ | |||||||||
HaTRP | Fusari et al. (2012) [17] | _ | _ | F: 5′ TTCTTTAGGCCAACCCTCAC 3’ | HanXRQChr16g0506311 | 40,982,842 | 40,983,492 | HanXRQChr16 | |
R: 5′ CCCTTAATCATAATTCACGAATGTC 3′ | |||||||||
HaWP | Fusari et al. (2012) [17] | _ | _ | F: 5′ CCAAACCCGATGATGATAAA 3′ | HanXRQChr10g0289571 | 69,322,685 | 69,323,065 | HanXRQChr10 | |
R: 5′ AACAAACAAAACAAGCCACATT 3′ | |||||||||
HaWRKY5 | Fusari et al. (2012) [17] | _ | _ | F: 5′ ACACCTCTCAACTTGCACCAAA 3′ | HanXRQChr17g0533851 | 2,017,423 | 2,019,342 | HanXRQChr17 | |
R: 5′ GCCCATGGATCTGAAGACAAA 3′ | |||||||||
HaWRKY7 | Fusari et al. (2012) [17] | _ | _ | F: 5′ CAAGCCGCTAGAGATGGAAC 3′ | HanXRQChr16g0509771 | 66,374,292 | 66,377,376 | HanXRQChr16 | |
R: 5′ TCAACCCTGTGGTGTTTTGA 3′ | |||||||||
HaRhobp_B | Fusari et al. (2012) [17] | _ | _ | F: 5′ TTGAGGGATTCTAATTGTTATAGTTGA3’ | ATROPGEF7 | 85,118,928 | 85,122,332 | HanXRQChr08 | |
R: 5′ TTCGGGTGTTCGTCCTTTT 3′ |