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Fig. 1 | BMC Plant Biology

Fig. 1

From: Inhibition of multiple defense responsive pathways by CaWRKY70 transcription factor promotes susceptibility in chickpea under Fusarium oxysporum stress condition

Fig. 1

Expression pattern of CaWRKY70 transcript and protein in chickpea. a Organ specific expression of CaWRKY70 in chickpea root, shoot, flower and pod tissues, respectively. CaWRKY70 mRNA levels in different organs were measured using CaGAPDH as internal control. Error bars represent mean ± SD of three independent biological replicates. bCaWRKY70 transcript levels after SA, ABA and JA treatments in susceptible JG62 and resistant WR315 chickpea shoots. CaGAPDH was used as internal control. Error bars represent ±SD (n = 3). Asterisks (*) indicate values that differ significantly from control treatment (0 dpi) as determined by Student’s t test (*P ≤ 0.05 and ***P ≤ 0.001). NS denotes not significant. c Normalized fold induction of CaWRKY70 transcript at shoot tissues of susceptible JG62 and resistant WR315 chickpea upon Foc1 infection. CaWRKY70 fold change values at different time-points are normalized against 0 dpi in JG62 and those in WR315, respectively. CaGAPDH expression was used as the reference control. Error bars represent ±SD values of three biological replicates. d CaWRKY70 protein accumulation was determined by western blotting with anti-CaWRKY70 antibody. Coomassie Brilliant Blue (CBB) stained gels serve loading control

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