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Fig. 1 | BMC Plant Biology

Fig. 1

From: CRISPR/Cas9-mediated mutation of OsSWEET14 in rice cv. Zhonghua11 confers resistance to Xanthomonas oryzae pv. oryzae without yield penalty

Fig. 1

Genetic modification of OsSWEET14 using CRISPR/Cas9. a Two targets were selected in the coding region of OsSWEET14. Target I is located in the 1st exon, while target II is located in the 3rd exon. Two pairs of primers (a and b, c and d) were used to amplify the two target regions for sequencing. Black boxes and white boxes represent the corresponding CDS and untranslated region of the OsSWEET14 transcript in the genome, respectively. a, Target-TalC-F; b, Target-TalC-R; c, Target-S14E3-F; and d, Target-S14E3-R. b Sequences of OsSWEET14 mutant alleles in the target regions. Mutations in CR-S14–2 and CR-S14–6 were homozygous in the T0 generation, while mutations in CR-S14–1, CR-S14–9, CR-S14–10 and CR-S14–29 were biallelic in the T0 generation. All the biallelic mutations were homozygous in the T1 or T2 generation. CR-S14–1-I was the same as CR-S14–6. Deletions and insertions are indicated by dashes and red letters, respectively. Target I and Target II are underlined in the wildtype (WT). The number of nucleotides that were deleted or inserted in mutant alleles is indicated on the right side of the mutant allele sequence

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