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Fig. 8 | BMC Plant Biology

Fig. 8

From: Comparative analyses of DNA repeats and identification of a novel Fesreba centromeric element in fescues and ryegrasses

Fig. 8

Co-localization of CENH3 with the Fesreba element in Festuca and Lolium. Combination of immunolocalization of the histone H3 variant CENH3 (red) and FISH on interphase nuclei with probes for: a the reverse transcriptase (RT) domain (green); and b the non-coding LTR part of the Fesreba element (green). F. pratensis cv. Fure (FPF); F. arundinacea subsp. arundinacea (FAR); F. mairei (FMA); L. multiflorum cv. Mitos (LMM). Column 1 shows merged images, column 2 shows CENH3 signals (red), and column 3 shows FISH signals corresponding to the Fesreba element. In all accessions, the signals of CENH3 and FISH probes are overlapping. Nuclei were counterstained with DAPI (blue). The bar corresponds to 10 μm

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