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Fig. 6 | BMC Plant Biology

Fig. 6

From: Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana

Fig. 6

qPCR analysis of transcript abundance regulation. a: Consistency test on the regulation for genes showing strong regulation at the end of the lag-phase (top), after cold triggering (middle) or after light triggering (bottom). The transcript levels were quantified with gene specific primers and standardized on the transcript level of YLS8 in 3–5 independently cultivated and treated biological replicates. The figure shows means ± standard deviation. Statistical significance of regulation (Student t-test; p < 0.05) is labelled with an asterisk. b: Testing for consistency of regulation of the RNASeq analysis throughout the experiment for three selected genes. For all samples, the transcript abundance ratio between primed and unprimed plants obtained by qPCR in four independently cultivated and treated biological replicates (green bars; means ± standard deviation) was in the range of the ratio calculated based on the FPKM-values of the RNASeq analysis (white bars)

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