Fig. 2
From: Cold priming uncouples light- and cold-regulation of gene expression in Arabidopsis thaliana
Effect of cold priming on light triggering. a Leaf surface temperature in the heat-filtered (orange) and in the not heat-filtered illumination set-up (red). b H2O2 content in control plants (white) and cold (blue) or high light triggered plants (orange). Different letters show statistical significance of differences based on data obtained with 9 plants from two independently cultivated and treated plant sets (Student t-test; p < 0.05). c Maximum quantum yield of photosystem II (FV/FM) in control plants (white) and cold (blue) or high light triggered plants (orange). The parameter was determined with a saturating white light flash after 20 min dark acclimation. Different letters show statistical significance of differences based on data obtained with 10 plants from two independently cultivated and treated plant sets (Student t-test; p < 0.05). d Relative transcript abundance of light-responsive genes in control plants (white) and after 2 h heat-controlled illumination (orange). The transcript levels were standardized on the transcript levels of YLS8; Statistically significant differences in the relative transcript abundances are labelled with asterisks (n = 3–5; Tukeys post hoc test; p < 0.05). e Relative transcript abundance of heat-responsive genes in control plants (white) and after 2 h heat-controlled illumination (orange) and not heat-controlled illumination (red). The transcript levels were standardized on the transcript levels of YLS8; Statistically significant differences in the relative transcript abundances are labelled with different letters (n = 3–5; Tukeys post hoc test; p < 0.05). f Effect of 24 h cold priming at 4 °C on transcript abundance regulation by a light stimulus. Regulation of the relative transcript abundances in control plants (C), only cold-primed (P), only light-triggered (T) and cold-primed + light-triggered plants (PT) immediately after light triggering. Different letters show statistical significance of differences in the relative transcript levels based on data obtained with 3–5 independently cultivated and treated biological replicates (Tukeys post hoc test; p < 0.05). All subfigures show means ± standard deviation