Fig. 5

Ploidy levels of nuclei isolated from leaf tissue. Figure shows percentages of nuclei populations extracted from leaf cells of Col-0, cpr5–2, SynCPR5 and metCPR5 plants. The nuclei were extracted from leaf cells of 24-old-plants and analysed by flow cytometry as described in Methods. Percentages of every population from each sample were calculated and average (percentage) values are shown in the graph. Each value represents the mean of three values from leaves of three different plants of the same line. Asterisks indicate significant difference from Col-0 at p < 0.05 (Student’s t-test)