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Fig. 2 | BMC Plant Biology

Fig. 2

From: Transcription factor TabHLH49 positively regulates dehydrin WZY2 gene expression and enhances drought stress tolerance in wheat

Fig. 2

Subcellular localization (a) and transactivation assay (b and c) of TabHLH49 protein. a Subcellular localization of TabHLH49 in tobacco epidermal cells. GFP and TabHLH49:GFP under the control of the CaMV35S promoter were separately transiently expressed in tobacco epidermal cells. Scale bar = 10 μm. b Diagram of the pGBKT7 construct that expressed different truncated TabHLH49 proteins in yeast. c Transactivation activities of the intact or truncated TabHLH49. Fusion proteins of the GAL4 DNA-binding domain and different portions of TabHLH49 were transformed into Y2H Gold cells and grown on SD/−Trp/X-α-gal/AbA plates to assess their transactivation activities

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