Skip to main content

Table 1 Results of the RT-qPCR analysis of the relative sp-Xeg gene expression level in the transgenic and control aspen lines

From: Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

Line Ct Actin Ct Ubiquitin Ct Xeg ∆ Ct ∆∆ Ct Relative sp-Xeg gene expression level
Pt (control) 29.2 ± 0.39 27.5 ± 0.85 30.5 ± 1.78 2.2 ± 2.10
Xeg-1-1a 30.1 ± 1.45 27.5 ± 0.77 19.1 ± 0.89 −9.7 ± 1.04 −1.4 2.6
Xeg-1-1b 29.9 ± 0.48 25.2 ± 0.33 18.2 ± 0.20 −9.4 ± 0.33 −1.1 2.1
Xeg-1-1c 30.6 ± 1.53 27.5 ± 0.85 18.5 ± 0.95 − 10.6 ± 1.11 −2.2 4.7
Xeg-2-1b 31.1 ± 0.68 27.9 ± 0.48 18.4 ± 0.06 − 11.1 ± 0.40 −2.7 6.7
Xeg-2-3b 28.1 ± 0.43 25.7 ± 0.38 17.3 ± 0.22 − 9.6 ± 0.34 −1.3 2.4
Xeg-2-5a 27.7 ± 0.22 24.4 ± 0.18 17.8 ± 0.30 −8.3 ± 0.23 0 1
\