Table 1 Results of the RT-qPCR analysis of the relative sp-Xeg gene expression level in the transgenic and control aspen lines
Line | Ct Actin | Ct Ubiquitin | Ct Xeg | ∆ Ct | ∆∆ Ct | Relative sp-Xeg gene expression level |
|---|---|---|---|---|---|---|
Pt (control) | 29.2 ± 0.39 | 27.5 ± 0.85 | 30.5 ± 1.78 | 2.2 ± 2.10 | – | – |
Xeg-1-1a | 30.1 ± 1.45 | 27.5 ± 0.77 | 19.1 ± 0.89 | −9.7 ± 1.04 | −1.4 | 2.6 |
Xeg-1-1b | 29.9 ± 0.48 | 25.2 ± 0.33 | 18.2 ± 0.20 | −9.4 ± 0.33 | −1.1 | 2.1 |
Xeg-1-1c | 30.6 ± 1.53 | 27.5 ± 0.85 | 18.5 ± 0.95 | − 10.6 ± 1.11 | −2.2 | 4.7 |
Xeg-2-1b | 31.1 ± 0.68 | 27.9 ± 0.48 | 18.4 ± 0.06 | − 11.1 ± 0.40 | −2.7 | 6.7 |
Xeg-2-3b | 28.1 ± 0.43 | 25.7 ± 0.38 | 17.3 ± 0.22 | − 9.6 ± 0.34 | −1.3 | 2.4 |
Xeg-2-5a | 27.7 ± 0.22 | 24.4 ± 0.18 | 17.8 ± 0.30 | −8.3 ± 0.23 | 0 | 1 |