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Fig. 3 | BMC Plant Biology

Fig. 3

From: ABC1K10a, an atypical kinase, functions in plant salt stress tolerance

Fig. 3

The abc1k10a mutants show increased sensitivity to salt stress.(a) Schematic diagram of the ABC1K10a gene. The filled boxes represent exons, while the lines between the boxes represent introns. Two T-DNA insertions are also indicated. (b) Transcript abundance of ABC1K10a in the wild type and abc1k10a mutants. Total RNAs isolated from 5 day-old wild type and 10a mutant seedlings were used for real-time qRT-PCR. Three biological replicates were conducted (means ± SD, n = 3). 10a, abc1k10a. Asterisks indicate statistical difference between 10a mutants and wild type (***P < 0.001, t-test). (c) Salt sensitivity of wild type and 10a mutants. Wild type, 10a-1 and 10a-2 mutants seeds were germinated and grown for 8 days on MS medium containing different concentrations of NaCl. Bar = 500 μm. (d) Cotyledon greening of seedlings grown on MS medium containing different concentrations of NaCl was scored after 10 days and represent averages of 100 seedlings from at least four independent experiments. (means ± SD; n = 100). Asterisks indicate statistically significant differences (**P < 0.01, t-test) between 10a mutants and wild type. (e) Cotyledon greening rates in wild type and 10a mutants in response to NaCl treatment. Wild type, 10a-1 and 10a-2 seeds were sown on MS medium or MS medium with 100 mM NaCl. Cotyledon greening rates were calculated at the indicated days. Error bars indicate ± SD (n = 100). Asterisks indicate statistically significant differences (***P < 0.001, t-test) between the 10a mutants and wild type. (F) The transcript levels of RD29A, KIN1 and COR15b in wild-type, 10a-1 and 10a-2 mutants at different times after treatment with NaCl. 5-day-old seedlings were treated without or with 200 mM NaCl at the indicated times, and transcript levels were analyzed by qRT-PCR. Error bars indicate ± SD (n = 3). Asterisks indicate statistically significant differences between the 10a mutants and wild type (**P < 0.01, ***P < 0.001, t-test)

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