Fig. 2

Molecular characterization of ABC1K10a. (a) GUS staining of transgenic Arabidopsis lines carrying a proK10a::GUS construct. b, e and f, bars = 2 mm. a, c and d, bars = 500 μm. (b) Expression of ABC1K10a in different tissues detected by qRT-PCR. ACTIN2/8 was used as a control. Asterisks indicate statistically significant difference between leaves and other tissues (**P < 0.01, t-test). (c) Schematic protein structure of ABC1K10a. MTS, mitochondrial targeting sequence; TM, transmembrane domain. (d) Subcellular localization of ABC1K10a (green fluorescence) and Mito-Tracker (red fluorescence) in Nicotiana benthamiana leaves. Bars = 50 μm. (e, f &g) Expression of ABC1K10a is induced by NaCl treatment. (E) Relative transcript levels of ABC1K10a (qRT-PCR) under salt stress were obtained by comparing treated and untreated samples. Error bars indicate ± SD (n = 3). Asterisks indicate statistically significant differences between treated and untreated samples at the different treatment times (**P < 0.01, t-test). (f) For GUS staining, 5 day-old transgenic seedlings expressing ABC1K10apro::GUS were treated with 200 mM NaCl for 0, 3 or 6 h. Bars = 200 μm. (g) To extract the total proteins, 5 day-old transgenic seedlings expressing ABC1K10a pro::ABC1K10a-GFP were treated with 200 mM NaCl for 0, 3 or 6 h. Protein extracts were analyzed by western blotting with α-GFP antibody (g). Actin was used as a control