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Table 2 Discrimination ability of standard and species-specific barcodes and their combinations

From: Complete plastome sequencing resolves taxonomic relationships among species of Calligonum L. (Polygonaceae) in China

DNA barcodes type

length (bp)

Polymorphic sites

Polymorphic sites (%)

Identified species (Success rate)

Standard DNA barcodes

nrITS

ITS1 + 5.8S

449

6

1.34

0 (0%)

5.8S + ITS2

467

16

3.43

1 (11%)

nrITS

768

22

2.92

1 (11%)

plastid

matK

1527

14

0.92

2 (22%)

rbcL

1428

8

0.56

2 (22%)

trnH-psbA

386

23

5.96

2 (22%)

combination

matK + rbcL

2955

22

0.74

3 (33%)

trnH-psbA + matK + rbcL

3341

44

1.32

3 (33%)

matK + rbcL + nrITS

3723

44

1.18

5 (56%)

trnH-psbA + matK + rbcL + nrITS

4109

66

1.61

4 (44%)

Genomic element

trnE-T

995

21

2.11

1 (11%)

trnT-L

1026

36

3.51

2 (22%)

potential specific-barcodes

ndhF

2244

38

1.69

5 (56%)

ndhF-rpl32

993

28

2.82

4 (44%)

rpl32-trnL

632

16

2.53

4 (44%)

trnC-petN

900

82

9.11

4 (44%)

trnS-G

1233

26

2.11

5 (56%)

Combination*

5795

200

3.45

6 (67%)

Complete plastid genomes (only one IR)

133,980

1151

0.86

7 (78%)