Table 2 Types of homozygous mutations identified in the T1 generation
From: Conversion of hulled into naked barley by Cas endonuclease-mediated knockout of the NUD gene
gRNA | Type of mutation | No. of plants | No. of transgenic plants / No. of transgene-free plants | NUD CDS reading frame |
|---|---|---|---|---|
Nud45 | −1 bp | 2 | 2/0 | Shifted |
−3 bp | 2 | 2/0 | Retained | |
− 214/+ 5 bp | 2 | 0/2 | Shifted | |
Nud50 | −1 bp | 8 | 6/2 | Shifted |
−3 bp | 6 | 4/2 | Retained | |
−6 bp | 5 | 5/0 | Retained | |
−17 bp | 8 | 6/2 | Shifted | |
−19 bp | 1 | 1/0 | Shifted | |
+ 1 bp (G) | 1 | 0/1 | Shifted |