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Table 2 Types of homozygous mutations identified in the T1 generation

From: Conversion of hulled into naked barley by Cas endonuclease-mediated knockout of the NUD gene

gRNA Type of mutation No. of plants No. of transgenic plants / No. of transgene-free plants NUD CDS reading frame
Nud45 −1 bp 2 2/0 Shifted
−3 bp 2 2/0 Retained
− 214/+ 5 bp 2 0/2 Shifted
Nud50 −1 bp 8 6/2 Shifted
−3 bp 6 4/2 Retained
−6 bp 5 5/0 Retained
−17 bp 8 6/2 Shifted
−19 bp 1 1/0 Shifted
+ 1 bp (G) 1 0/1 Shifted