Table 1 Analysis of leaf samples taken from T0 plants with cas9/gRNA expression units being stably integrated
From: Conversion of hulled into naked barley by Cas endonuclease-mediated knockout of the NUD gene
gRNA | Genotype | Phenotype | ||||||
|---|---|---|---|---|---|---|---|---|
transgenic T0 plants | mutated plants | homozygous mutations | Efficiency (%) | Plants with grains | only hulled grains | hulled and naked grains | only naked grains | |
Nud45 | 46 | 35 | 8 | 76 | 40 | 7 | 27 | 6 |
Nud50 | 48 | 20 | 0 | 42 | 43 | 22 | 16 | 5 |