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Table 1 Analysis of leaf samples taken from T0 plants with cas9/gRNA expression units being stably integrated

From: Conversion of hulled into naked barley by Cas endonuclease-mediated knockout of the NUD gene

gRNA Genotype Phenotype
transgenic T0 plants mutated plants homozygous mutations Efficiency (%) Plants with grains only hulled grains hulled and naked grains only naked grains
Nud45 46 35 8 76 40 7 27 6
Nud50 48 20 0 42 43 22 16 5