Fig. 5
Bimolecular fluorescence complementation validation of the bio-interaction of OsSHMT and ATP synthase subunit α, ATP synthase subunit β, Hsp70, MSCP and APX from rice. To validate the positive interaction of OsSHMT with ATP synthase α subunit (ATP-synα), ATP synthase β subunit (ATP-synβ), heat shock protein 70, mitochondrial substrate carrying family (MSCP) protein E and ascorbate peroxidase (APX) in rice, their genes were amplified from Dular rice and respectively infused with the N-terminal or C-terminal of YFP to construct YFPn- and YFPc-containing recombinant vectors for bimolecular fluorescence complementation (BiFC), according to transient transformation in the leaves of Nicotiana benthamiana. The YFP fluorescence was detected using laser confocal microscopy under 488-nm excitation light