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Fig. 4 | BMC Plant Biology

Fig. 4

From: Serine hydroxymethyltransferase localised in the endoplasmic reticulum plays a role in scavenging H2O2 to enhance rice chilling tolerance

Fig. 4

Protein interactions with OsSHMT in Arabidopsis thaliana. The recombinant vector of OsSHMT fused with GFP was used to genetically transform Arabidopsis thaliana and T3 homozygous transgenic lines were taken to isolate proteins interacting with OsSHMT. A vector containing only GFP without OsSHMT was used as a control to transform into A. thaliana to obtain the transformed lines. Natural leaf proteins from OsSHMT transgenic A. thaliana and GFP transgenic A. thaliana were respectively extracted and incubated with GFP-Trap agarose beads to reveal the proteins interacting with OsSHMT

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