Fig. 3

Proteins binding on the OsSHMT-promoter in Dular and Lsi1-OX rice and the differences in transcription level between the two types of rice. The promoter region of OsSHMT was amplified using the specific primers with biotin labelled at the 5′ end and then fused with Streptavidin-coupled Dynabeads and natural leaf proteins from Dular or Lsi1-OX rice. The protein and DNA complex mixture was extracted and incubated with OsSHMT promoter-containing Dynabeads and fished using a magnetic frame to collect the proteins and then separated by SDS-PAGE (a). qPCR was conducted to determine the change in the transcription levels of AA-ATPase, histone H1, nucleic acid binding protein (NABP) and tubulin/FtsZ domain containing protein (LOC_Os03g51600, LOC_Os05g34170, LOC_Os07g38730) for Lsi1-OX and Dular rice under chilling treatment of different durations (d)