Fig. 2

Relative expression of OsSHMT in the Lsi1-OX rice line and Dular rice (a) and subcellular co-localisation of OsSHMT protein with endoplasmic reticulum marker protein (b). Changes in the gene expression level of OsSHMT before chilling treatment and after treatment for 12, 24 and 36 h were compared for Lsi1-OX and Dular rice (a). OsSHMT was fused with eYFP and inserted into pCambia 2300 to construct the recombinant vector for OsSHMT expression and detect subcellular protein localisation. The recombinant vector was transformed into rice protoplast for transient expression, together with a mcherry fused ER localised protein as a marker. Yellow fluorescence and mcherry fluorescence were respectively detected and then merged to validate the OsSHMT subcellular localisation (b)