Fig. 1

Phenotypic changes in the leaves under chilling treatment (a), subcellular localisation of Lsi1 driven by ubiquitin promoter (b) and SEM images of the leaf surface of Dular and Lsi1-OX (c). The Dular rice and the transformed Lsi1-OX line were treated at 4 °C for 36 h and the phenotypic changes in the leaves of the two rice lines were compared (a). Lsi1 was fused with GFP and inserted into a modified pCambia 1301 vector in which the Lsi1 gene was transcribed by the ubiquitin promoter. The recombinant Lsi1 expression vector was transformed in the rice protoplast and subcellular localisation of OsNIP2;1 was detected using laser scanning confocal microscopy (b). SEM images of the surfaces of the two rice leaves were compared to determine the differences in silica deposition (c)