Fig. 7From: Melatonin may increase disease resistance and flavonoid biosynthesis through effects on DNA methylation and gene expression in grape berriesTranscription capacity of the EDS1 promoter to drive GUS expression in the presence of melatonin and/or MET1. a Expression difference of the five selected genes in ‘Merlot’ berries at 48 HAT from RNA-Seq and qRT-PCR analyses. MET1, VIT_212s0035g01770; MET1B, VIT_212s0035g01755; SadMET, VIT_214s0006g02170; CMT2_1; VIT_216s0039g02470; CMT2_2, VIT_216s0039g02460. b Prediction of methylated cytosine in the EDS1 promoter (http://www.urogene.org/cgi-bin/methprimer2). O/E values indicate the ratio between the actual value and expected value of the CpG locus. The 800-bp region with a high CpG level indicated by the black box was used as the promoter of EDS1 (Peds) to produce the construct of Peds-35S miniGUS. c Histochemical analysis of the transcriptional capacity of Peds to drive GUS expression in grape calluses agroinfiltrated with different vector constructs: B1, control calluses; B2 and B4, Peds-35S miniGUS; B3, Peds-35S miniGUS and 35S::MET1; B5, Peds-35S miniGUS and 35S::MET1 with melatonin treatment; B6, Peds-35S miniGUS with melatonin treatment. d Gus activities of grape calluses infiltrated by Agrobacterium containing the B1-B6 constructs. e DNA methylation level of the EPS1 promoter, including endogenous DNA and DNA provided by Peds- 35S miniGUS, in calluses infiltrated by Agrobacterium containing the B4-B6 constructsBack to article page