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Fig. 7 | BMC Plant Biology

Fig. 7

From: Structural and functional similarities and differences in nucleolar Pumilio RNA-binding proteins between Arabidopsis and the charophyte Chara corallina

Fig. 7

Restoration analyses of the salt- and sugar-sensitive apum23 and apum24 phenotypes by ChPUM2 and ChPUM3. aapum23–2 (left panel) and apum24–1+/− (right panel) seedlings expressing either 35S:ChPUM2 or 35S:ChPUM3 in the absence or presence of NaCl and glucose. Col-0 Control (pB2GW7), apum24–1+/−, 35S:ChPUM2/apum24–1+/−, and 35S:ChPUM3/apum24–1+/− seeds were germinated on 1/2 MS medium containing 10 mg L− 1 Basta with the indicated treatment. b Expression levels of APUM24 in Col-0 Control (pB2GW7), apum24–1+/−, and transgenic apum24–1+/− plants expressing 35S:ChPUM2 and 35S:ChPUM3 in the absence and presence of 200 mM glucose. Note the similar expression levels of APUM24 in the presence of 200 mM glucose. Original gel images are provided in Additional file 5: Figure S5. c qRT-PCR analysis of the relative unprocessed rRNA levels in Col-0 Control (pB2GW7), apum24–1+/−, and transgenic apum24–1+/− plants expressing 35S:ChPUM2 and 35S:ChPUM3 in the presence of 200 mM glucose. The same primers were used as in Fig. 5. Two technical and three biological replicates were performed for PCR measurements. Values represent means ± SDs (n = 3).

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