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Fig. 6 | BMC Plant Biology

Fig. 6

From: Structural and functional similarities and differences in nucleolar Pumilio RNA-binding proteins between Arabidopsis and the charophyte Chara corallina

Fig. 6

Complementation assays of 35S:ChPUM2 and 35S:ChPUM3 transgenic plants in the apum24+/− mutant background. a T-DNA insertion site of apum24–1 mutant alleles (upper panel) and genotyping (bottom panel). Primers used for genotyping are indicated with arrows. Original gel images for bottom panel are provided in Additional file 4: Figure S4a. b Confirmation of the expression of ChPUM2 and ChPUM3 transgenes in the apum24–1+/− mutant using RT-PCR. Original gel images are provided in Additional file 4: Figure S4b. c Siliques of Col-0 control, apum24–1+/−, and transgenic apum24–1+/− expressing 35S:ChPUM2 or 35S:ChPUM3. The right panels for each plant line are enlarged images of the boxed regions. Arrows and arrowheads indicate undeveloped ovules and aborted seeds, respectively. Note that none of the transgenics complemented the abnormal seeds to normal levels. d qRT-PCR for analyzing relative unprocessed rRNA levels in Col-0 control, apum24–1+/−, and 35:ChPUMN/apum24–1+/− using the same primers that were used in Fig. 5. Two technical and three biological replicates were performed for PCR measurements. Values represent means ± SDs (n = 3) (**; p < 0.01)

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