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Fig. 5 | BMC Plant Biology

Fig. 5

From: Structural and functional similarities and differences in nucleolar Pumilio RNA-binding proteins between Arabidopsis and the charophyte Chara corallina

Fig. 5

Complementation assays of 35S:ChPUM2 and 35S:ChPUM3 transgenic plants in the apum23 mutant background. a Confirmation of the expression of ChPUM2 and ChPUM3 transgenes in the apum23–2 mutant using RT-PCR. Gel images were processed from original figures (Additional file 3: Figure S3) by Photoshop CS5. b Normal germination and root growth of apum23–2 complemented with 35S:ChPUM2.c Plant heights and rosette leaves in mature plants. Leaves were collected from 2-week-old plants. d Recovery of streptomycin susceptibility in the apum23–2 complemented 35S:ChPUM2.e qRT-PCR analysis for unprocessed rRNAs in wild-type Col-0, apum23–2, 35S:ChPUM2/apum23–2, and 35S:ChPUM3/apum23–2. Two technical and three biological replicates were performed for PCR measurements. Asterisks indicate the results of Student’s t-test between apum23–2 and transgenic plants (**; p < 0.01). Values represent means ± standard deviations, SDs (n = 3). The left panel shows a schematic diagram of poly(A) pre-rRNA byproducts and the primers used for the detection of pre-rRNAs. Unprocessed poly(A) 18S (~ 2.6 knt) and 5.8S (~ 300 nt) pre-rRNAs are shown below the 35S pre-rRNA

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