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Table 2 fatty acid composition of tobacco leaf from wild type and fad2–2 mutant plants

From: Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9-mediated knockout of NtFAD2–2

Vector Fatty acid composition (mol%)
16:0 18:0 18:1Δ9 18:2Δ9, 12 18:3Δ9, 12, 15
WT 12.6 ± 0.2 4.9 ± 0.1 6.1 ± 0.1 12.2 ± 0.2 64.1 ± 1.7
fad2–2#06 12.7 ± 0.3 4.5 ± 0.2 6.3 ± 0.2 12.7 ± 0.1 63.8 ± 1.2
fad2–2#14 11.9 ± 0.2 5.1 ± 0.3 5.8 ± 0.1 13.1 ± 0.3 64.8 ± 2.1
  1. 16:0, palmitic acid; 18:0, stearic acid; 18:1Δ9, oleic acid; 18:2Δ9, 12, linoleic acid; 18:2Δ9, 12, 15, linolenic acid. Data are means ± SD of three independent experimental replicates