Fig. 5

CRISPR-Cas9 mediated NtFAD2–2 genes mutation. a, Targeting site and gRNA sequence used for NtFAD2–2 genes editing. 19 bp gRNA sequence was underlined. The 3 base PAM sequence was in red. b, Sequences of wild type and representative mutation types induced at the targeting site of NtFAD2–2 were presented, respectively. c, DNA sequencing peaks of WT and representative mutation types at the targeting site of NtFAD2–2, respectively. The red arrows indicate the location of mutations