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Fig. 7 | BMC Plant Biology

Fig. 7

From: Genome-wide identification and characterization of cucumber bHLH family genes and the functional characterization of CsbHLH041 in NaCl and ABA tolerance in Arabidopsis and cucumber

Fig. 7

CsbHLH041 transgenic Arabidopsis showed enhanced salt and ABA tolerance. (a) Relative expression of CsbHLH041 in Col-0 (WT) and two T3 generation transgenic lines by semi-quantitative PCR. The actin8 gene was used as an internal control. The original, uncropped gel image was provided as Additional file 9. (b) Germination of WT seeds of Col-0 and CsbHLH041 transgenic lines OX-1, OX-2 on 1/2 MS supplemented with 100 mM NaCl and 2 μM ABA after 7 days of cultivation at 22 °C. (c) and (d) Seed germination rate for the corresponding (b), respectively. Three biological replications were performed. Asterisks indicated a significant difference **p < 0.01 compared with the corresponding controls. The growth of Col-0 (WT) and CsbHLH041 transgenic lines after 200 mM NaCl (e) and 100 μM ABA (f) treatments

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