Fig. 7From: Genome-wide identification and characterization of cucumber bHLH family genes and the functional characterization of CsbHLH041 in NaCl and ABA tolerance in Arabidopsis and cucumberCsbHLH041 transgenic Arabidopsis showed enhanced salt and ABA tolerance. (a) Relative expression of CsbHLH041 in Col-0 (WT) and two T3 generation transgenic lines by semi-quantitative PCR. The actin8 gene was used as an internal control. The original, uncropped gel image was provided as Additional file 9. (b) Germination of WT seeds of Col-0 and CsbHLH041 transgenic lines OX-1, OX-2 on 1/2 MS supplemented with 100 mM NaCl and 2 μM ABA after 7 days of cultivation at 22 °C. (c) and (d) Seed germination rate for the corresponding (b), respectively. Three biological replications were performed. Asterisks indicated a significant difference **p < 0.01 compared with the corresponding controls. The growth of Col-0 (WT) and CsbHLH041 transgenic lines after 200 mM NaCl (e) and 100 μM ABA (f) treatmentsBack to article page