Fig. 3

Structural analyses of fixed and sectioned wheat grains using light microscopy (a-h, 26 dpa) and scanning electron microscopy of freeze fractured grains (i and j; 21 and 28 dpa respectively). Control (on the left, a, c, e, g, i) and homozygous AsWRI1-wheat line with multiple insert (on the right, b, d, f, h, j). Grain sections stained with MAS staining proteins green and starch in dark purple a-d and Sudan black staining lipids in dark e-h. Al; aleurone cells, SE; starchy endosperm, SAl; subaleurone cells. Scale bars 100 μm a, b, e, f, 20 μm c, d, g, h and 50 μm i, j