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Fig. 4 | BMC Plant Biology

Fig. 4

From: Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts

Fig. 4

Sequence characterization and phylogenetic analysis. a. Multiple sequence alignment of the deduced amino acid sequences of F3’H from ‘Er Qiao’ cDNA and six other plant species was conducted using DNAMAN software. Sequences used are from A. thaliana (AF271651), Callistephus chinensis (AF313488), Glycine max (AF499731), Matthiola incana (AF313491), Petunia hybrida (AF155332), and Sorghum bicolor (AAV74195). b. Phylogenetic tree showing the evolutionary relatedness of ‘Er Qiao’ F3’H with that of other plants. The accession numbers of the included F3’H proteins are as follows: P. hybrida (X71130), Antirrhinum majus (AB028151), Gentiana triflora (BAD91808), Ipomoea purpurea (AAR00229), Matthiola incana (AAG49301), Vitis vinifera (BAE47006), Delphinium grandiflorum (AAX51796), Eustoma exaltatum (BAD34460), Vinca major (BAC97831), Paeonia suffruticosa (HQ283449), and Paeonia suffruticosa (KJ466967). The tree was constructed using the neighbor-joining method in MEGA5 software. Numbers along the branches indicate bootstrap support determined from 1000 repetitions, and the bar indicates an evolutionary distance of 0.02%

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